BSc-1 Year Biotechnology syllabus 

In university B.Sc.-I year The syllabus of BIOTECHNOLOGY consists of two papers of theory and one paper of practical’s.

PAPER – I

BIOCHEMISTRY, BIOSTASTICS AND
COMPUTERS

UNIT-I

1. Introduction
to Biochemistry: History, Scope and Development.

2. Carbohydrates:
Classification, Structure and Function of Mono, Oligo and Polysaccharides.

3. Lipids:
Structure, Classification and Function.

UNIT –II

1. Amino acids
and Proteins: Classification, Structure and Properties of amino acids, Types of Proteins and their
Classification and Function.

2. Enzymes:
Nomenclature and Classification of enzyme, Mechanism of enzyme action, Enzyme Kinetics and Factors
affecting the enzymes action. Immobilization of enzyme and their application.

UNIT –III

1. Hormones:
Plant Hormone-Auxin and Gibberellins and Animal Hormone-Pancreas and Thyroid.

2. Carbohydrates,
Proteins and Lipid Metabolism – Glycolysis, Glycogenesis,

Glyconeogenesis,
Glycogenolysis and Krebs cycle. Electron Transport Chain and β[1]oxidation of Fatty
acids.

UNIT-IV

1. Scope of
Biostatistics, Samples and Population concept, Collection of data-sampling techniques, Processing and
Presentation of data.

2. Measures of
Central Tendency: Mean, Median and Mode and Standard Deviation.

3. Probability
Calculation: Definition of probability, Theorem on total and compound probability.

UNIT-V

1. Computers –
General introduction, Organization of computer, Digital and Analogue

Computers and Computer
Algorithm.

2. Concept of
Hardware and Software, Input and Output Devices.

3. Application
of computer in co-ordination of solute concentration, pH and Temperature etc., of a Fermenter in operation
and Internet application

B.Sc.-I year BIOTECHNOLOGY

PAPER–II

CELL BIOLOGY, GENETICS AND
MICROBIOLOGY

UNIT-I

1. Concept of
life, Cell as a basic unit of living system and Cell theory.

2. Diversity of
Cell shape and size.

3. Prokaryotic
cell structure: Function and ultra structure of cell (Gram positive and Gram negative Bacteria), Plasma
membrane, Flagella, Pilli, Endospore and Capsule.

4. Eukaryotic
cell: Plant cell wall and Plasma membrane.

UNIT-II

1. Cytoplasm:
Structure and Functions of Endoplasmic reticulum, Ribosome, Golgi

complex, Lysosomes, Nucleus,
Mitochondria and Chloroplast.

2. Cytoskeleton:
Microtubules, Microfilaments and Intermediate filaments.

3. Cell
division: Mitosis and Meiosis.

4. Programmed
Cell Death..

UNIT-III

1. Mendel’s Laws
of Inheritance.

2. Linkage and
Crossing over.

3. Chromosome
variation in number and structure: Deletion, Duplication, Translocation, Inversion and Aneuploidy,
Euploidy (Monoploidy and Polyploidy and its importance).

UNIT-IV

1. History,
Scope and Development of Microbiology.

2. Basic
techniques of Microbial Culture

3. Microbial
Growth & Nutrition of Bacteria: Isolation, media sterilization- physical
and chemical agents, pure
culture-pour plate method, streak plate method and spread plate method.

4. General
features and Economic importance of Fungi, Algae and Protozoa etc.

UNIT-V

1. Bacterial
Reproduction: Conjugation, Transduction and Transformation.

2. Mycoplasma –
History, Classification, Structure reproduction & Diseases.

3. Viruses –
Basic features, Structure, Classification, Multiplication, Bacteriophages

 (Morphology, life cycle,
infection and medicinal importance)

List of Practical’s

MICROBIOLOGY AND BIOCHEMICAL
TECHNIQUES

(1) Laboratory
rules, Tools, Equipment and Other requirements in Microbiological laboratory.

(2) Micrometry –
Use of ocular & stage Micrometrer.

(3) Counting of
bacteria by counting chamber, by plate count.

(4)Preparation
of media and cultivation techniques:

• (a) Basic
  liquid media (broth)
• (b) Basic
  Solid media, (agar slants and deep tubes)
• (c)
  Demonstration of selective and differential media
• (d) Isolation
  and enumeration of micro organisms
• (e) Isolation
  from air and Soil

(5)Smears and
staining methods:

• (a)
  Preparation of bacterial smear
• (b) Gram
  Negative & Positive staining

(6)Methods of
obtaining pure cultures

• (a) Streak
  plate method
• (b) Pure plate
  method
• (c) Spread
  plate method
• (d) Broth
  cultures

(7)Growth &
Biochemical techniques

• (a)
  Determination of bacterial growth curve
• (b) Amylase
  production test
• (c) Cellulose
  production test
• (d) Estimation
  of Sugar in given solution
• (e) Extraction
  and separation of lipids
• (f) Estimation
  of proteins
• (h) Mitosis
  and Meiosis

(8)Biostatistics:

• (a) By Manual
  and by computer.
• (b) Problems
  on mean, mode and median

SCHEME OF PRACTICAL
EXAMINATION

Time – 4
hrs. M. M.: 50

• 1. Experiment
  based on culture of micro-organisms 15 Marks
• 2. Bacterial
  growth/Staining techniques 10 Marks
• 3. Biochemical
  techniques 05 Marks
• 4. Bio
  statistics 05 Marks
• 5. Spotting 05
  Marks
• 6. Viva – Voce 05
  Marks
• 7. Record/Sessional
  05 Mark